Group G/9 resistant Lolium perenne from New Zealand

International Survey of Herbicide-Resistant Weeds

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Sunday, June 16, 2019

GROUP G/9 RESISTANT PERENNIAL RYEGRASS
(Lolium perenne)

EPSP synthase inhibitors (G/9)

New Zealand

INTRODUCTION		PERENNIAL RYEGRASS
Perennial Ryegrass (Lolium perenne) is a monocot weed in the Poaceae family. In New Zealand this weed first evolved resistance to Group G/9 herbicides in 2012 and infests Grapes. Group G/9 herbicides are known as EPSP synthase inhibitors (Inhibition of EPSP synthase). Research has shown that these particular biotypes are resistant to glyphosate and they may be cross-resistant to other Group G/9 herbicides. The 'Group' letters/numbers that you see throughout this web site refer to the classification of herbicides by their site of action. To see a full list of herbicides and HRAC herbicide classifications click here.

QUIK STATS (last updated Aug 16, 2013 )
Common Name	Perennial Ryegrass
Species	Lolium perenne
Group	EPSP synthase inhibitors (G/9)
Herbicides	glyphosate
Location	New Zealand
Year	2012
Situation(s)	Grapes
Contributors - (Alphabetically)	Kerry Harrington
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NOTES ABOUT THIS BIOTYPE

GENERAL

Kerry Harrington

For the perennial ryegrass populations, we have three separate populations, and the level of resistance in each is 30-fold, 13-fold and 10-fold respectively. The PhD student who is lead author of this paper is currently working on mode of action and genetic studies for these populations, and has just about finished a quick cross-resistance study.

A published paper describing this case can be found at:

http://www.nzpps.org/journal/66/nzpp_660890.pdf

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ACADEMIC ASPECTS

Confirmation Tests

Field, Greenhouse, and Laboratory trials comparing a known susceptible Perennial Ryegrass biotype with this Perennial Ryegrass biotype have been used to confirm resistance. For further information on the tests conducted please contact the local weed scientists that provided this information.

Genetics

Genetic studies on Group G/9 resistant Perennial Ryegrass have not been reported to the site. There may be a note below or an article discussing the genetics of this biotype in the Fact Sheets and Other Literature

Mechanism of Resistance

The mechanism of resistance for this biotype is either unknown or has not been entered in the database. If you know anything about the mechanism of resistance for this biotype then please update the database.

Relative Fitness

There is no record of differences in fitness or competitiveness of these resistant biotypes when compared to that of normal susceptible biotypes. If you have any information pertaining to the fitness of Group G/9 resistant Perennial Ryegrass from New Zealand please update the database.

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CONTRIBUTING WEED SCIENTISTS

KERRY HARRINGTON

Massey University 624 Betts Rd Palmerston North New Zealand Email Kerry Harrington

ACKNOWLEDGEMENTS
The Herbicide Resistance Action Committee, The Weed Science Society of America, and weed scientists in New Zealand have been instrumental in providing you this information. Particular thanks is given to Kerry Harrington for providing detailed information.

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Herbicide Resistant Perennial Ryegrass Globally
(Lolium perenne)

Country

FirstYear

Situation

Active Ingredients

Site of Action

Argentina

2008

Soybean, Spring Barley, and Wheat

glyphosate

EPSP synthase inhibitors (G/9)

Chile

2001

Cereals

clodinafop-propargyl, and diclofop-methyl

ACCase inhibitors (A/1)

Denmark

2016

Wheat

clodinafop-propargyl, and iodosulfuron-methyl-sodium

Multiple Resistance: 2 Sites of Action
ACCase inhibitors (A/1)
ALS inhibitors (B/2)

Germany

2008

Wheat

iodosulfuron-methyl-sodium, pinoxaden, and pyroxsulam

Multiple Resistance: 2 Sites of Action
ACCase inhibitors (A/1)
ALS inhibitors (B/2)

New Zealand

2012

Grapes

glyphosate

EPSP synthase inhibitors (G/9)

New Zealand

2015

Grapes

amitrole, glufosinate-ammonium, and glyphosate

Multiple Resistance: 3 Sites of Action
Carotenoid biosynthesis (unknown target) (F3/11)
EPSP synthase inhibitors (G/9)
Glutamine synthase inhibitors (H/10)

Portugal

2013

Grapes

glyphosate

EPSP synthase inhibitors (G/9)

United States (California)

1989

Railways, and Roadsides

sulfometuron-methyl

ALS inhibitors (B/2)

United States (Texas)

1989

Roadsides

sulfometuron-methyl

ALS inhibitors (B/2)

Specific Papers about this Case

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Ghanizadeh, H. ; Harrington, K. C. ; James, T. K. ; Woolley, D. J.. 2013. Confirmation of glyphosate resistance in two species of ryegrass from New Zealand vineyards. New Zealand Plant Protection 66 : 89 - 93.

Plants were obtained from two populations of Italian ryegrass (Lolium multiflorum) and three populations of perennial ryegrass (Lolium perenne) from different vineyards in Marlborough and Nelson that were suspected of being resistant to glyphosate following many consecutive applications of this herbicide over recent years. Each population was multiplied by splitting out tillers, and this was also done for plants taken from a population of each species from Manawatu pastures where they had not been exposed to glyphosate application. A dose-response experiment showed that four populations taken from the vineyards were about 10 times as resistant to glyphosate as those plants that had not been previously exposed to the herbicide. The experiment was repeated and showed one perennial ryegrass population to have a 30-fold level of resistance. These are the first confirmed cases of glyphosate resistance within New Zealand..

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Literature about Similar Cases

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Menegat, A., G. C. Bailly, R. Aponte, G. M. T. Heinrich, B. Sievernich, and R. Gerhards. 2016. Acetohydroxyacid synthase (AHAS) amino acid substitution Asp376Glu in Lolium perenne: effect on herbicide efficacy and plant growth.. J Plant Dis Prot DOI 10.1007/s41348-016-0023-2 : .

Recently, the acetohydroxyacid synthase (AHAS) amino acid substitution Asp376Glu was detected in a Lolium perenne population originating in France. This is the first documented occurrence of the Asp376Glu single- nucleotide polymorphism in a Lolium species. The effects on herbicide efficacy and plant fitness are presented. Separation of the original population into different genetic subgroups allowed the comparison of different L. perenne AHAS genotypes according to their susceptibility to AHAS-inhibiting herbicides. Root and shoot biomass accumulation as well as tiller production was analysed for resistant and sensitive populations. Genotype-specific AHAS activity and inhibition were studied in vitro, using recombinant Arabidopsis thaliana DNA. Plants expressing the AHAS Asp376Glu genotype were resistant against mesosulfuron ? iodosulfuron, pyroxsulam and propoxycarbazone. To imazamox, reduced susceptibility for dosages below 140 g a.i. ha-1 was observed. In the absence of herbicide, no significant impact of the Asp376Glu genotype on shoot biomass and tiller accumulation could be observed, whereas root biomass of the Asp376Glu genotype was significantly reduced by 68 % compared to the AHAS genetic wild type. The AHAS enzyme study revealed reduced AHAS enzyme rate of 48 % for the Asp376Glu genotype compared to the AHAS genetic wild type. This study highlights the role of the amino acid exchange on resistance profile against the most relevant AHAS-inhibiting herbicides. First indications were found that fitness of the Asp376Glu genotype is hampered..

Salas, R. A. ; Burgos, N. R. ; Mauromoustakos, A. ; Lassiter, R. B. ; Scott, R. C. ; Alcober, E. A.. 2013. Resistance to ACCase and ALS inhibitors in Lolium perenne ssp. multiflorum in the United States. Journal of Crop and Weed 9 : 168 - 183.

Lolium perenne ssp. multiflorum (Italian ryegrass) is a major weed problem in wheat production. Being an obligate outcrossing species, it has a high propensity to evolve resistance to herbicides. This study was conducted to determine the level of resistance and resistance patterns of Italian ryegrass populations in the United States to ACCase- (diclofop and pinoxaden) and ALS (imazamox, mesosulfuron, and pyroxsulam) inhibitors. Dose-response bioassays were conducted on 47 populations from suspect herbicide-resistant fields in the southern United States (Arkansas, Mississippi, Georgia, North Carolina, South Carolina, Virginia), Kansas, and Washington collected between 2008 and 2011 from fields suspected of resistance to ALS inhibitors. Eighty-seven percent of the populations were resistant to diclofop. Eight of the diclofop-resistant populations (25%) were also resistant to pinoxaden. Thirty-eight diclofop-resistant populations (81%) were resistant to at least one ALS inhibitor. Thirty-nine populations (83%) were resistant to mesosulfuron, 38 of which were also resistant to pyroxsulam. All mesosulfuron-resistant populations tested were cross-resistant to imazamox and pyroxsulam. Cross-resistance patterns to ALS inhibitors differed. Of 45 populations with resistance to ACCase or ALS inhibitors, 35 (78%) had multiple resistance to both modes of action. The majority of diclofop-resistant L. perenne populations can be controlled with pinoxaden, but widespread resistance to pinoxaden can evolve soon if it is intensively used. A comprehensive weed management approach prior to planting will be critical in managing L. perenne in wheat and preventing or delaying resistance evolution..

Wu JinXia ; Zhang ZhiGuo ; Zhang Qian ; Lang ZhiHong ; Sun XueHui. 2012. Scarabaeid larvae- and herbicide-resistant transgenic perennial ryegrass (Lolium perenne L.) obtained by Agrobacterium tumefaciens-mediated transformation of cry8Ca2, cry8Ga and bar genes. Journal of Integrative Agriculture 11 : 53 - 61.

Insect pest and weeds are two major problems for forage and turf grasses. In this study, scarab larvae- and herbicideresistant transgenic perennial ryegrass (Lolium perenne L.) was obtained by transforming it with cry and bar genes simultaneously via the Agrobacterium-mediated method. To optimize the callus induction and plant regeneration conditions, various concentrations of 2,4-dichlorophenoxyacetic acid and 6-benzylaminopurine were assayed. The transformation efficiencies of different Agrobacterium suspension media, used during Agrobacterium-mediated transformation, were compared. Then, plasmids of pCAMBIA3301 containing cry gene (cry8Ca2 or cry8Ga) and bar gene, driven by ubiquitin promoter, were transformed into perennial ryegrass. The transformants were generated and confirmed by both Southern hybridization analysis and Western hybridization analysis. Further, the resistance of transgenic perennial ryegrass plants to scarab larvae and herbicide were analyzed. After 30 d of co-cultivation with scarab larvae, the damage to the root system of transgenic plants was less than that of non-transgenic control plants. Additionally, the leaves of transgenic plants were resistant to Basta®, while leaves of the wild plants wilted after Basta® spraying. These results show that cry gene and bar gene were successfully transferred into perennial ryegrass by the Agrobactgerium-mediated method, and convey resistance to scarab larvae and herbicide in transgenic perennial ryegrass plants..

Baldwin, C. M. ; Brede, A. D. ; Mayer, J. J.. 2012. 'JS501' and 'Replay' perennial ryegrass glyphosate tolerance and rates required for annual bluegrass (Poa annua L.) control. HortScience 47 : 932 - 935.

Incorporating the use of glyphosate into a weed management program offers turfgrass managers increased flexibility and cost savings when attempting to control troublesome weeds such as annual bluegrass (ABG) (Poa annua L.). Field trials of glyphosate tolerant perennial ryegrass (PRG) (Lolium perenne L.) cultivars, JS501 and Replay, were initiated to determine glyphosate tolerance and rates required for ABG control. In the tolerance trial, glyphosate was applied on 15 Sept. 2010 and 9 Aug. 2011 at rates of 0, 0.29, 0.58, 1.16, 1.74, 2.32, and 3.48 kg.ha^-1 a.e. Glufosinate was also applied at 0, 1.68, and 3.37 kg.ha^-1 a.i. In the ABG control trial, glyphosate was applied on 17 June followed by 19 Aug. 2009 and 25 June followed by 25 Aug. 2010 at rates of 0, 0.15, 0.29, 0.44, and 0.58 kg.ha^-1 a.e. In the tolerance trial, linear regression analysis revealed a glyphosate application rate of 0.81 kg.ha^-1 a.e. was required to cause 20% leaf firing. By the end of the trial, the highest rate of glufosinate resulted in nearly complete desiccation of 'Replay' PRG. For ABG control, after four glyphosate applications over a 2-year period, a rate of 0.29 kg.ha^-1 a.e. or greater resulted in less than 10% ABG. Untreated plots had ~83% ABG infestation. Discoloration was not noted for either PRG cultivar at any point over the 2-year trial period. Based on the environmental conditions of each trial, results suggest a recommended application rate should be 0.29 kg.ha^-1 a.e. during summer months. This rate is sufficient for ABG control and also provides protection in case spray overlap occurs during an application..

Salas, R. A. ; Dayan, F. E. ; Pan, Z. Q. ; Watson, S. B. ; Dickson, J. W. ; Scott, R. C. ; Burgos, N. R.. 2012. EPSPS gene amplification in glyphosate-resistant Italian ryegrass (Lolium perenne ssp. multiflorum) from Arkansas. Pest Management Science 68 : 1223 - 1230.

BACKGROUND: Resistance to glyphosate in weed species is a major challenge for the sustainability of glyphosate use in crop and non-crop systems. A glyphosate-resistant Italian ryegrass population has been identified in Arkansas. This research was conducted to elucidate its resistance mechanism. RESULTS: The investigation was conducted on resistant and susceptible plants from a population in Desha County, Arkansas (Des03). The amounts of glyphosate that caused 50% overall visual injury were 7 to 13 times greater than those for susceptible plants from the same population. The EPSPS gene did not contain any point mutation that has previously been associated with resistance to glyphosate, nor were there any other mutations on the EPSPS gene unique to the Des03 resistant plants. The resistant plants had 6-fold higher basal EPSPS enzyme activities than the susceptible plants, but their I₅₀ values in response to glyphosate were similar. The resistant plants contained up to 25 more copies of EPSPS gene than the susceptible plants. The level of resistance to glyphosate correlated with increases in EPSPS enzyme activity and EPSPS copy number. CONCLUSION: Increased EPSPS gene amplification and EPSPS enzyme activity confer resistance to glyphosate in the Des03 population. This is the first report of EPSPS gene amplification in glyphosate-resistant Italian ryegrass. Other resistance mechanism(s) may also be involved..

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